Epstein-Barr Virus (HHV-4) Research

What is Epstein-Barr Virus (HHV-4) ?

HHV-4  structure
Picture 1 HHV-4 moving through blood cells

HHV-4 Includes a dual cell tropism for Individual B-lymphocytes (generally non-productive disease ) and epithelial cells (productive infection). There’s absolutely no acceptable animal host, but replication/latency has been studied extensively in transformed human cell lines. HHV-4 is widespread worldwide, with ~75% of adults infected (lifelong! ) ) .

Virions Enveloped; slightly pleomorphic; curved; 120-220 nm in diameter. Surface projections of envelope small (surface appears rough); spikes; dispersed evenly over all of the surface. Nucleocapsids isometric. Nucleocapsid surrounded by the tegument which consists of globular material which is frequently asymmetrically distributed and may be variable in quantity. Nucleocapsids sometimes penetrated by stain (although undamaged envelope impermeable to blot ); 100-110 nm in diameter. Symmetry icosahedral. Nucleocapsids appear to be angular. Surface capsomer arrangement obvious. 162 capsomers a nucleocapsid (capsomeres hexagonal in cross-section with a hole running halfway down the long run ). Core is made up of fibrillar spool on which the DNA is wrapped. Incomplete virus particles often present; they’re capsids lacking the envelope.

Virions Contain one molecule of linear double stranded DNA.

Total genome length is 170000 nt. Genome sequence has terminal repeated sequences; Reiterated, but not replicated internally; replicated at both ends. Guanine + Cytosine ratio 56%. Each virion contains full length copy, or briefer Duplicates (some isolates deficiency up to 15000 at particular sites).

Biotech products available for EBV research:

1.      EBV Assays and Components

Virusys Corporation’s EBV qPCR 20x probe combination is a trusted probe for measuring comparative amounts or complete copy amounts of EBV by qualitative PCR. The probe contains reverse and forward primers along with a FAM/BHQ-labeled probe specific to BALF5 (DNA polymerase) of EBV. This dual-labeled probe is provided with PCR primers in 20x concentration and might be utilized with regular real time PCR reagents.

For complete copy quantity calculations, a tube of criteria can be included containing the target sequence at 1012 copies per watt. These criteria produce linear outcomes at dilutions of 108 to 102 copies per response.

2.     EBV Antigen Slides

Antigen slides are particularly suited to immunofluorescence but may also be used in immunohistochemical protocols. Infected cells are combined with uninfected cells so that every of the slide has”built in” unwanted controls. Employed with our optimized lineup of pre-mixed secondary cells, the antigen reveals itself through glowing green fluorescence against a dark red background of uninfected cells.

Each slide has eight separate wells set in a hydrophobic Teflon mask. Reagents that are incubated in the infected cells sit as a”bead” on the and the Teflon keeps the liquid out. They are supplied in packages of five individually packaged slides and full directions for use.

Epstein-Barr Virus Antigen Slide ive, eight-well slips with chemically induced human B lymphocytes expressing Epstein-Barr virus (P3HR1)

From where could we supply detection products in Europe?

It is quite simple you could contact Gentaur, Virusys partner for EU. Please select the contact from the list below and you would get an offer within an hour:
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