Rat VF(Visfatin) ELISA Kit

Description: serum, plasma, tissue homogenates or other biological fluids.

Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Visfatin (VF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Visfatin (VF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Visfatin (VF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Visfatin (VF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Rat Visfatin (VF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VF in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VF in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.094 ng/ml

Description: serum, plasma, tissue homogenates and other biological fluids.